Genome Analysis In Farm Animals

October 30, 2020
Genome Analysis In Farm Animals

Traditionally, The Genetics Of Livestock Has Been Based On ThePhenotypic Characteristics Of Animals. However, The Explosion InOur Understanding Of Mammalian Genome And AccompanyingTechnological Innovations Has Opened Up New Avenues In Farm AnimalGenetic Research. Advances In Molecular Genetics During The LastTwo Decades Have Been Phenomenal And The Researchers Are MoreInclined To Use GenomicTools To Solve Their Research Questions.Molecular Markers Especially DNA Markers That Are Capable OfRevealing Genetic Variations At DNA Level Are Now Key Players InAnimal Genetic Research Programs. Various DNA Markers LikeRestricted Fragment Length Polymorphism (RFLP), Short TandemRepeats (Microsatellites), Single Nucleotide Polymorphisms (SNP),Etc. And DNA Sequencing Methods Are Increasingly Used ByResearchers Not Only For Marker Based Genetic Improvement ProgramsBut Also In Conservation Genetics To Analyze The Diversity AndRelationship Of Livestock Populations. These DNA Markers PossessUnique Genetic Properties Which Help Researchers Overcome TheLimitations Of Classical Genetic Markers Like Morphological,Biochemical And Protein Markers. However, There Are Only LimitedLiteratures That Deal With The Ways And Means Of Analyzing GeneticMarkers With Special Emphasis On Farm Animals. The Present Book IsAn Attempt In This Direction To Compile The Theoretical InformationAnd Practical Methods Available For Genome Analysis Of DomesticAnimals. The Book Has Three Main Sections: Part I Deal With DNAMarkers Available For Genome Analysis In Farm Animals. The BasicPrinciples And Properties Of Various DNA Markers Like RFLP,Microsatellites, Snps, Etc. And Their Applications In LivestockHave Been Dealt In Detail In The First Four Chapters While TheMethods Of Gene Mapping And Differential Gene Expression AnalysisAre Dealt In The Fifth And Sixth Chapters. Part II Focusses OnPractical Aspects Of Different Tools Used For Genome Analysis OfFarm Animals Including Preparation Of Metaphase Chromosomes,Physical Mapping Using Fluorescent In Situ Hybridization,Polymerase Chain Reaction, Agarose And Polyacrylamide GelElectrophoresis, DNA Sequencing, Real Time PCR Applications, Etc.Part III Gives A Brief Overview On Different Bioinformatics ToolsWith Special Emphasis On Designing Oligonucleotide Primers AndAnalysis Of DNA Sequences Generated In The Laboratory. Our Aim IsTo Provide Readers The Theoretical Background And Practical StepsInvolved In The Analysis Of Most Commonly Used DNA Markers InLivestock. We Hope That This Book Has Useful Information PertinentTo The Needs Of Students And Researchers Of Farm Animal GeneticResearch.